ABSTRACT
Objective To learn the HPV infection situation of gynecological patients ,so as to provide a scientific basis for the epidemiological studies in Chongqing area .Methods Totally 12 196 gynecology clinic patients in January 2010 to December 2013 were registered whose cervical secretions was collected as specimens ,was type testing was carried on by PCR‐reverse dot hybridiza‐tion ,age distribution ,infected type and multiple infection of the patients were counted .Results There were 3 745(30 .8% ) patients with a positive test specimen of 12 196 patients detected in this study ,the high‐risk HPV infection rate was 61 .67% ,the top three high‐risk HPV subtype were HPV58 ,HPV16 and HPV52 ;the low‐risk HPV infection rate was 21 .23% and the main subtypes were HPV6 and HPV43 .The majority of multiple infections was double infection .There were significant differences between each age groups (χ2 =129 .76 ,P<0 .05) ,the paragraph of HPV infection rate present as U shaped curve .Conclusion The HPV in‐fection of gynecology clinic patients in Chongqing area has obvious regional specificity and age differences .HPV58 ,HPV16 and HPV52 should be pay attention in clinical diagnosis .The age group ≤25 and ≥46 years old are two peaks of HPV infections ,and it should be strengthen the screening of the two age groups .
ABSTRACT
Objective To evaluate the perfusion parameters in different zone of placenta using CPS,to research placental blood perfusion of different zone. Methods A total of 60 pregnant rats were divided into 3groups, 15 day,17 day and 20 day of gestation with 20 animals in each group,the placenta was divided into the central zone and marginal zone. SonoVue was injected by tail vein of rat using CPS, the time-intensity curves of the central zone and marginal zone were drawn and perfusion paramcters were calculate by software ACQ and Sonoliver. Surface area densities of different zone of placental maternal blood space was measured with histoligical method,and using immunohistochemical marked the placenta vascular. Results Arrivel time(AT) and time-to-peak(TTP) of placenta central zone was earlier than marginal zone,the peak intensity(PI) of the central zone was higher than that of the marginal zone ( P ＜ 0.05). There was significant difference between the central zone and marginal zone of surface area densities of placental maternal blood space ( P ＜0.05). PI of the central zone and marginal zone and the surface area densites of placenta was positively correlated ( P ＜0.05). The placental blood vessels was not expressed by factor Ⅷ.Laminin in the placental basement membrane expression of 20 day of gestation was not continuous or missing. Conclusions The surface area densities of central zone and marginal zone was different. CPS technique can be sensitive to detect changes of the placenta vascular bed in different zone.
ABSTRACT
Objective To quantitatively measure the perfusion parameters of placenta in different stage of pregnant rats using contrast-enhanced ultrasonography (CEUS) with contrast pulsed sequencing (CPS), and to analyze the relationship between perfusion parameters and changes of placental vascular bed. Methods Sixty healthy pregnant rats in according to the requirements of the experiment was divided into three groups: 15 days, 17 days and 20 days of gestation with 20 animals in each group. One blous injection of SonoVue (Sonovue 1.0 ml/kg) via a tail vein was administered to each rat, and the time-intensity curves (TIC) of placenta and uterine muscle wall were drawn with ACQ using CPS technique with MI 0.20, and the perfusion parameters were calculated. Then 4 μm vertical placenta sections were cut and stained with hematoxylin and eosin. Surface area densities of placental maternal blood space was measured with image analysis software. Results The peak intensity (PI) of 17 days and 20 days was higher than that of 15 days pregnant rats (P＜0.05). There was no difference in PI between 17 days and 20 days (P＞0.05), and nor of arrivel time (AT) and time-to-peak (TTP) (P＞0.05) among the three groups. There was significant difference of surface area densities of placental maternal blood space among the three groups (P＜0.05). PI was positively correlated to the surface area densites of placenta (P＜0.05). Conclusion There is close relationship between peak intensity and area densities of placental maternal blood space. CPS technique can sensitivly detect changes of the placenta vascular bed in different stage of pregnant rats.